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Identification of Associated Proteins by Coimmunoprecipitation Protocol - Details

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Protocols » Listing Details
ID:2300
Title: Identification of Associated Proteins by Coimmunoprecipitation Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3898
Description:Coimmunoprecipitation is most commonly used to test whether two proteins of interest are associated in vivo, but it can also be used to identify novel interacting partners of a target protein. In both cases, the cells, which may have been labeled with [35S]methionine, are harvested and lysed under conditions that preserve protein-protein interactions. The target protein is specifically immunoprecipitated from the cell extracts, and the immunoprecipitates are fractionated by SDS-PAGE.
Category:Immunology: Immunoprecipitation Protocols: Immunoprecipitation of Radiolabeled Cells Protocols
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Date Added:December 22, 2007 07:54:14 AM
Number Hits:172
 

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