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Detection of Protein Phosphorylation in Tissues and Cells Protocol - Details

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Protocols » Listing Details
ID:2299
Title: Detection of Protein Phosphorylation in Tissues and Cells Protocol - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&objectId=6677CD53C088A0E7A7B22F422FFCDC12&treeNodeId=9E6639B7FED0C828BBFE860A8E957694
Description:Most powerful and convincing method to determine if a specific protein is phosphorylated in a physiologically relevant manner is to assay phosphorylation in situ. The procedure described involves incubating cultured cells (e.g., primary neuronal cultures or transfected cells) or tissue preparations (e.g., hippocampal slices) with [32P]orthophosphate, which is then taken up by the cells or tissues and incorporated into the γ-phosphate position of ATP.
Category:Immunology: Immunoprecipitation Protocols: Immunoprecipitation of Radiolabeled Cells Protocols
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Date Added:December 22, 2007 07:53:01 AM
Number Hits:16
 

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