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Chromatin Immunoprecipitation (ChIP) of Protein Complexes Protocol - Details

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Protocols » Listing Details
ID:2296
Title: Chromatin Immunoprecipitation (ChIP) of Protein Complexes Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4560
Description:Protocol describes the use of chromatin immunoprecipitation technology (ChIP) to analyze interactions of proteins or protein complexes with DNA in vivo. In this approach, the material is fixed with formaldehyde to preserve DNA-protein and protein-protein associations, the cells are lysed, and the chromatin is cut and solubilized. The chromatin suspension is immunoprecipitated with an antibody against the protein(s) of interest, and the coimmunoprecipitated DNA fragments are analyzed.
Category:Immunology: Immunoprecipitation Protocols: Chromatin Immunoprecipitation Protocols
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Date Added:December 22, 2007 07:46:11 AM
Number Hits:113
 

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