Procedure permits the isolation of at least 5 µg of total RNA from a sample of purified mouse splenic B lymphocytes. The quality of the RNA is assessed by separation of an aliquot through 1% agarose and staining with ethidium bromide as described in AfCS protocol Visualization of RNA
Preparations on 1% Agarose Gels. The isolated RNA is used for analysis of gene expression by microarray technology.
analysis of gene expression by microarray technology.