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Isolation of Resting B Lymphocytes from One or More Groups of Four Mouse Spleens Protocol - Details

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Protocols » Listing Details
ID:2272
Title: Isolation of Resting B Lymphocytes from One or More Groups of Four Mouse Spleens Protocol - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000001.pdf?pid=PP00000001
Description:Describes the isolation of resting B lymphocytes (B cells) from mouse spleens by using negative selection with anti-CD43 and anti-Mac-1/CD11b monoclonal antibodies coupled to magnetic microbeads. This strategy depletes non-B cells from a mixed population of splenocytes and relies on the fact that most mature leukocytes, with the exception of resting splenic B cells, express CD43.
Category:Immunology: B Cell Protocols
Link Owner:
Date Added:December 20, 2007 01:32:07 PM
Number Hits:48
 

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