Proliferative Assays for T Cell Function Protocols- https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=9E6634679E96E622003E15187F105306&objectid=6674B7F5E219FD4021F448C7D13AF27A
Description:
Protocols describe the conditions required to induce proliferation are described. Also describe the assay in which CD4·CD25·T cells are co-cultured with conventional T cells in order to assess their suppressive function. Will describe the culture conditions for the activation and expansion of CD4·CD25· cells.