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Production of Mouse T Cell Hybridomas Protocol - Details

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Protocols » Listing Details
ID:2265
Title: Production of Mouse T Cell Hybridomas Protocol - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=9E663496BA646F5C4FB54D6064E1A19B&objectid=6674C004CF35A9AC863EAD85BD9F080A
Description:T cell hybridomas can be obtained by fusing activated T cells with tumor cells. A heterogeneous population of hybridomas can be cloned by limiting dilution to obtain hybridomas that express specificity to one T cell receptor (TCR). Protocol describes cell fusion and selection of T cell hybridomas. A protocol is provided for screening of T cell hybridomas for expression of the CD3-TCR complex by flow cytometry analysis.
Category:Immunology: T Cell Protocols
Link Owner:
Date Added:December 20, 2007 01:11:48 PM
Number Hits:25
 

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