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Immunohistochemistry with AP-Conjugated (NBT/BCIP) Protocol - Details

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Protocols » Listing Details
ID:2230
Title: Immunohistochemistry with AP-Conjugated (NBT/BCIP) Protocol - http://icg.cpmc.columbia.edu/cattoretti/Protocol/immunohistochemistry/NBTBCIPstaining.html
Description:Protocol for immunohistochemistry with AP-Conjugated (NBT/BCIP). Protocol extensively blocks slides, further diluting the primary antibody, lengthening the incubation and washing time, using a simple AP-conjugated secondary at high dilution and use a slow long development with the most powerful IHC development, NBT/BCIP. Includes: Single AP stainiing and Double AP staining.
Category:Immunohistochemistry Protocols
Link Owner:
Date Added:December 19, 2007 07:50:29 PM
Number Hits:198
 

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