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Antigen Retrieval for Immunohistochemistry with Paraffin-Embedded Tissues Protocol - Details

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Protocols » Listing Details
ID:2207
Title: Antigen Retrieval for Immunohistochemistry with Paraffin-Embedded Tissues Protocol - http://www.animal.ufl.edu/hansen/Protocols/Immunohistochemistry_paraffin.htm
Description:Protocol used to for immunohistochemistry on paraffin-embedded sections. Based on use of microwave energy to effect antigen retrieval. The immunohistochemistry procedure, is for use of Biomeda's HistoScan kit based on a streptavidin-peroxidase/biotinylated second antibody detection system with 3-amino, 9-ethylcarbazole (AEC) as chromogen. Undoubtedly, other kits or home-made reagents will also work .
Category:Immunohistochemistry Protocols: Antigen Retrieval Protocols
Link Owner:
Date Added:December 17, 2007 06:53:51 PM
Number Hits:436
 

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