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Labeling Antibodies with Iodine Protocol - Details

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Protocols » Listing Details
ID:2155
Title: Labeling Antibodies with Iodine Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/18/pdb.prot4287
Description:This protocol describes a simple chemical oxidation method for labeling antibodies with iodine. Iodide-125 (supplied as NaI) is oxidized to form iodine-125 (I2), which attacks tyrosyl and histidyl side chains. The iodinated antibodies are easily detected and quantitated using gamma counters or film. They are used primarily in immunoassays, but other techniques can be adapted conveniently to the iodine detection method.
Category:Antibody Protocols: Antibody Labeling Protocols
Link Owner:
Date Added:December 16, 2007 11:20:47 PM
Number Hits:68
 

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