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Labeling Antibodies with Biotin Protocol - Details

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Protocols » Listing Details
ID:2153
Title: Labeling Antibodies with Biotin Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/18/pdb.prot4286
Description:This protocol describes the covalent coupling of antibodies to biotin. Biotin groups bind with extremely high affinity to streptavidin and avidin, both of which are available commercially coupled with enzymes, fluorescent dyes, or iodine. A biotinylated primary antibody, therefore, can be detected with any of a wide variety of different labels. The biotinylation reaction is simple and mild, and rarely inactivates the antibody.
Category:Antibody Protocols: Antibody Labeling Protocols
Link Owner:
Date Added:December 16, 2007 11:16:26 PM
Number Hits:301
 

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