Bioinformatics, Protocols, DNA RNA Protein Proteomics
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Link Details
| ID: | 2153 |
| Title: | Labeling Antibodies with Biotin Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/18/pdb.prot4286 |
| Description: | This protocol describes the covalent coupling of antibodies to biotin. Biotin groups bind with extremely high affinity to streptavidin and avidin, both of which are available commercially coupled with enzymes, fluorescent dyes, or iodine. A biotinylated primary antibody, therefore, can be detected with any of a wide variety of different labels. The biotinylation reaction is simple and mild, and rarely inactivates the antibody. |
| Category: | Antibody Protocols: Antibody Labeling Protocols |
| Link Owner: | |
| Date Added: | December 16, 2007 11:16:26 PM |
| Number Hits: | 46 |
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