Bioinformatics, Protocols, DNA RNA Protein Proteomics
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Link Details
| ID: | 2151 |
| Title: | Binding Antibodies to Tissue Sections Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4331 |
| Description: | Once tissues are fixed and permeabilized, the antibodies are added. These antibodies can be labeled directly or detected by a labeled secondary reagent. For indirect detection, any reagent that binds specifically to the primary antibody can be "tagged" and used to locate the antibody. The possible reagents include anti-immunoglobulin antibodies, protein A or G, or, if the first antibody is labeled with biotin, streptavidin. They can be labeled with enzymes or gold. |
| Category: | Antibody Protocols: Antibody Labeling Protocols |
| Link Owner: | |
| Date Added: | December 16, 2007 11:13:10 PM |
| Number Hits: | 20 |
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