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Binding Antibodies to Tissue Sections Protocol - Details

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Protocols » Listing Details
ID:2151
Title: Binding Antibodies to Tissue Sections Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4331
Description:Once tissues are fixed and permeabilized, the antibodies are added. These antibodies can be labeled directly or detected by a labeled secondary reagent. For indirect detection, any reagent that binds specifically to the primary antibody can be "tagged" and used to locate the antibody. The possible reagents include anti-immunoglobulin antibodies, protein A or G, or, if the first antibody is labeled with biotin, streptavidin. They can be labeled with enzymes or gold.
Category:Antibody Protocols: Antibody Labeling Protocols
Link Owner:
Date Added:December 16, 2007 11:13:10 PM
Number Hits:104
 

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