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Epitope Mapping Using Synthetic Biotin-Labeled Peptides Protocol - Details

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Protocols » Listing Details
ID:2150
Title: Epitope Mapping Using Synthetic Biotin-Labeled Peptides Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/18/pdb.prot4278
Description:A set of overlapping synthetic peptides is synthesized, each corresponding to a small segment of the linear sequence of a protein antigen and arrayed on a solid phase. The panel of solid-phase peptides is then probed with a test antibody, and bound antibody is detected using an enzyme-labeled secondary antibody. This method is very rapid and can be extraordinarily successful.
Category:Antibody Protocols: Epitope Mapping Antibody Protocols
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Date Added:December 16, 2007 11:11:56 PM
Number Hits:23
 

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