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Epitope Mapping by Competition Assay Protocol - Details

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Protocols » Listing Details
ID:2149
Title: Epitope Mapping by Competition Assay Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/18/pdb.prot4277
Description:The simplest way to determine whether two monoclonal antibodies bind to distinct sites on a protein antigen is to carry out a competition assay. The assay can be used with antibodies that bind both conformational and linear epitopes, and it is most useful in the analysis of monoclonal antibody specificity because polyclonal sera typically recognize multiple different epitopes.
Category:Antibody Protocols: Epitope Mapping Antibody Protocols
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Date Added:December 16, 2007 11:10:51 PM
Number Hits:192
 

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