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Gene Delivery by Direct Injection (Microinjection) Using a Pulsed-Flow System Protocol - Details

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Protocols » Listing Details
ID:2122
Title: Gene Delivery by Direct Injection (Microinjection) Using a Pulsed-Flow System Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/30/pdb.prot4653
Description:This protocol describes a method for pulsed-flow microinjection using the Eppendorf FemtoJet injector and Eppendorf InjectMan; this is the most common type of pulsed-flow microinjection system currently being used. The advantage of this type of system over a controlled-flow system is that much more control is available over the injection parameters, reducing variability in injections. In addition, the system allows a diagonal insertion of the needle into the cell.
Category:Microinjection Protocols: Microinjection of DNA Protocols
Link Owner:
Date Added:December 16, 2007 08:18:50 PM
Number Hits:37
 

 

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