Special Feature

User Panel

My Panel

My Panel

Bookmark Science Articles

Recent News
Bookmark / Share This Science Site

Send Us Your Protocols

Calibration of Micropipette Tips Protocols - Details

Link Details 

Protocols » Listing Details
ID:2111
Title: Calibration of Micropipette Tips Protocols - http://www.cshprotocols.org/cgi/content/abstract/2007/1/pdb.prot4655
Description:This protocol describes an easy method for calibrating micropipette tips that have been pulled in the laboratory. It is essential to estimate the internal diameter of the pulled micropipette tip when adjusting parameters for a new puller or new type of glass tubing. A tip diameter of ~0.3 µm is optimal for the microinjection of mammalian cells in culture (e.g., CHO, PtK1, and COS-7). A 10% increase in diameter increases the delivery rate by more than 30% and can cause cell damage.
Category:Microinjection Protocols: Microinjection Equipment
Link Owner:
Date Added:December 16, 2007 08:02:30 PM
Number Hits:198
 

Protocols Tags

  • activity Protocols
  • blocking Protocols
  • bochner Protocols
  • cardiac Protocols
  • cdna Protocols
  • cell Protocols
  • cloning Protocols
  • cryo Protocols
  • culture Protocols
  • describes Protocols
  • dna Protocols
  • electron Protocols
  • electropharyngeogram Protocols
  • endogenous Protocols
  • epg Protocols
  • golgi Protocols
  • gus Protocols
  • health Protocols
  • heart Protocols
  • immunogold Protocols
  • includes Protocols
  • intact Protocols
  • interphase Protocols
  • isolation Protocols
  • light Protocols
  • lots Protocols
  • membranes Protocols
  • microscopy Protocols
  • miniature Protocols
  • mitochondrial Protocols
  • myocytes Protocols
  • nuclei Protocols
  • one Protocols
  • page Protocols
  • plant Protocols
  • preparation Protocols
  • purification Protocols
  • rig Protocols
  • safety Protocols
  • sds Protocols
  • selection Protocols
  • serum Protocols
  • staining Protocols
  • strand Protocols
  • studies Protocols
  • synthase Protocols
  • synthesis Protocols
  • tail Protocols
  • tets Protocols
  • tissue Protocols


    •  

    Molecular Station Menu

    Welcome to Molecular Station!

    You have to register before you can post on our forums or use our advanced features. Register Now! Its Free and Fast!

    Already registered? Login now below.

    User Name:

    Password:

    Already registered and Forgot your password? Click below to recover it.

    Recover Lost Password

    Home
    Features

    Protocols

    DNA Forum

    Science Forum

    DNA Forum
    Biology Forum