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PEI Nanoparticles for Targeted Gene Delivery Protocol - Details

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Protocols » Listing Details
ID:2109
Title: PEI Nanoparticles for Targeted Gene Delivery Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/1/pdb.prot4451
Description:This protocol describes the preparation of polyethylenimine (PEI)/DNA nanoparticles for targeted gene delivery. This delivery strategy improves the efficiency of gene transfer by enhancing the entry of gene vectors into the desired cells and reducing uptake by nontarget cells. We describe here methods for the conjugation of targeting peptides to PEIs, formation of DNA complexes using the conjugated PEIs or nonconjugated PEIs together with targeting peptides, and cell transfection.
Category:Gene Delivery Protocols: DNA Nanoparticles Protocols
Link Owner:
Date Added:December 16, 2007 07:52:29 PM
Number Hits:84
 

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