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Bioresponsive Targeted Charge Neutral Lipid Vesicles for Systemic Gene Delivery Protocol - Details

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Protocols » Listing Details
ID:2107
Title: Bioresponsive Targeted Charge Neutral Lipid Vesicles for Systemic Gene Delivery Protocol - http://www.cshprotocols.org/cgi/content/short/2006/1/pdb.prot4450
Description:This protocol describes a stepwise procedure to prepare nucleic acids encapsulated in a polyethylene glycol (PEG)-shielded nanolipoparticle (NLP) that contain a bioresponsive lipid and ligand. This process provides several advantages for systemic gene delivery. The in vivo circulation time is extended. Also, low pH-sensitive lipids enhance DNA unpacking and endosomal escape. Finally, ligands inserted into the NLP surface can target gene delivery to specific tissues or cells in vivo.
Category:Gene Delivery Protocols: DNA Nanoparticles Protocols
Link Owner:
Date Added:December 16, 2007 05:44:14 PM
Number Hits:16
 

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