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Clonality - X Chromosome Inactivation Assay Protocol - Details

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Protocols » Listing Details
ID:2100
Title: Clonality - X Chromosome Inactivation Assay Protocol - http://cgap-mf.nih.gov/Protocols/DNARNAProteomicAnalysis/DNA/DNAProtocols/Clonality.html
Description:Investigators can utilize X chromosome inactivation (methylation) to determine the clonality status of a tumor or premalignant lesion in females. The technique is based on a methylation-sensitive restriction enzyme and analysis of a polymorphic locus on the X chromosome. Clonal cell populations will show "loss" of the non-methylated allele after restriction digest. The assay can be performed on DNA recovered from microdissected samples. Both frozen tissue and fixed-embedded tissue can be used.
Category:Genetics and Genomics: Cancer Genetics Protocols
Link Owner:
Date Added:December 16, 2007 05:14:00 PM
Number Hits:28
 

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