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A Multiplex PCR Method to Define a Narrow Deleted Chromosomal Region of a Tumor Genome - Details

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Protocols » Listing Details
ID:2092
Title: A Multiplex PCR Method to Define a Narrow Deleted Chromosomal Region of a Tumor Genome - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4117
Description:DNA for analysis is purified using salt precipitation. The method is gentle, limits the breakage of the long chromosomal strands, and avoids the use of phenol and chloroform. It is suitable for use with cultured cells, breast tumor tissue that has been subjected to hormone receptor analysis, and blood samples. The loss of heterozygosity assay is performed using a multiplex PCR, in which one of each primer pair is labeled with a different fluorophor.
Category:Genetics and Genomics: Cancer Genetics Protocols: Loss of Heterozygosity Protocols
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Date Added:December 16, 2007 05:01:27 PM
Number Hits:78
 

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