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Generation of Sets of Nested Deletion Mutants with Exonuclease III Protocol - Details

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Protocols » Listing Details
ID:2078
Title: Generation of Sets of Nested Deletion Mutants with Exonuclease III Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4078
Description:The double-stranded DNA of recombinant plasmid, phagemid, or bacteriophage M13 replicative form DNA is digested with two restriction enzymes whose sites of cleavage both lie between one end of the target DNA and the binding site for universal primer. The enzyme that cleaves nearer the target sequence must generate either a blunt end or a recessed 3' terminus; the other enzyme must generate a four-nucleotide protruding 3' terminus.
Category:Genetics and Genomics: Genotyping Protocols: Mutation Detection Protocols
Link Owner:
Date Added:December 16, 2007 03:50:26 PM
Number Hits:12
 

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