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Direct Selection of cDNAs with Large Genomic DNA Clones Protocol - Details

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Protocols » Listing Details
ID:2076
Title: Direct Selection of cDNAs with Large Genomic DNA Clones Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4077
Description:The goal of this method is to identify transcriptionally active genes in cloned segments of genomic DNA. The protocol uses hybridization and affinity purification to recover biotin-labeled cDNAs that bind to a 500-kb segment of human DNA cloned in a BAC vector. However, the method can be easily adapted to other clones of genomic DNAs cloned in high-capacity vectors.
Category:Genetics and Genomics: Genotyping Protocols: Mutation Detection Protocols
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Date Added:December 16, 2007 03:48:09 PM
Number Hits:6
 

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