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Combined DNA In Situ Hybridization and Immunocytochemistry Protocol - Details

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Protocols » Listing Details
ID:2055
Title: Combined DNA In Situ Hybridization and Immunocytochemistry Protocol - http://www.roche-applied-science.com/PROD_INF/MANUALS/InSitu/pdf/ISH_127-134.pdf
Description:Protocol for combined DNA in situ hybridization and immunocytochemistry for the simultaneous detection of nucleic acid sequences, proteins, and incorporated BrdU in cell preparations. Includes: Cell preparations and BrdU labeling; Detection of antigen by immunocytochemistry (ICC); Visualization of ICC antigen; -Gal-BCIG reaction (for producing a blue precipitate visible under brightfield microscopy); Cell processing for in situ hybridization; In situ hybridization (ISH); etc...
Category:Cytogenetics Protocols: In Situ Hybridization Protocols
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Date Added:December 15, 2007 12:59:42 PM
Number Hits:158
 

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