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Fluorescent In Situ Transcription in Cells and Tissues Protocol - Details

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Protocols » Listing Details
ID:2048
Title: Fluorescent In Situ Transcription in Cells and Tissues Protocol - https://catalog.invitrogen.com/index.cfm?fuseaction=iProtocol.unitSectionTree&treeNodeID=9E6639A7FEDD7A49BBC3758516F99557&objectid=6677C9E8B913BFB72E9F654025F3C39A
Description:Method assesses cellular mRNA transcripts in tissue sections and cell cultures using unique short anti-sense primers directed against sequences in particular protein(s). The unlabeled synthetic cDNA oligonucleotide primers are extended complementary to a sense mRNA transcript using reverse transcriptase and labeled through incorporation of a fluorescent-labeled dUTP nucleotide base. This procedure provides rapid detection of low abundance mRNA messages that can be related to other cellular....
Category:Cytogenetics Protocols: Fluorescent In Situ Hybridization FISH Protocol
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Date Added:December 15, 2007 12:39:23 PM
Number Hits:234
 

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