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Visualizing miRNAs in Adult Skeletal Muscle by Hybridization in situ Protocol - Details

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Protocols » Listing Details
ID:2030
Title: Visualizing miRNAs in Adult Skeletal Muscle by Hybridization in situ Protocol - http://www.natureprotocols.com/2006/06/29/visualizing_mirnas_in_adult_sk.php
Description:Protocol for visualizing miRNAs in adult skeletal muscle by hybridization in situ. Includes: Visualizing miRNAs in adult skeletal muscle by hybridization in situ; Probe preparation; Tissue preparation; Fixation; Deproteinization with Proteinase K; Acetylation of the tissue; Dehydration; Hybridization step; Post-Hybridization Washes (Step 1-6 should be performed on shaker); Signal Detection (Steps should be performed on a shaker); Post-Detection Washes.
Category:Cytogenetics Protocols: RNA In Situ Hybridization Protocols
Link Owner:
Date Added:December 15, 2007 11:25:56 AM
Number Hits:48
 

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