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Detection of mRNAs on Cryosections of the Cardiovascular System Using DIG-Labeled RNA Probes - Details

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Protocols » Listing Details
ID:2025
Title: Detection of mRNAs on Cryosections of the Cardiovascular System Using DIG-Labeled RNA Probes - http://www.roche-applied-science.com/PROD_INF/MANUALS/InSitu/pdf/ISH_189-196.pdf
Description:Protocol for detection of mRNAs on cryosections of the cardiovascular system using DIG-labeled RNA probes. Protocol was optimized from a protocol using 35S-labeled RNA probes. It allows to detect the expression of low abundant mRNAs in the cardiovascular system, e.g. of the proinflammatory cytokine GM-CSF in normal human coronary arteries, and of IL6 and gp130 in human failing hearts. The protocol can be combined with immunohistochemistry.
Category:Cytogenetics Protocols: In Situ Hybridization Tissue Sections Protocols
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Date Added:December 14, 2007 10:30:17 PM
Number Hits:66
 

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