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Identification of Single Bacterial Cells using DIG-Labeled Oligonucleotides Protocol - Details

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Protocols » Listing Details
ID:2012
Title: Identification of Single Bacterial Cells using DIG-Labeled Oligonucleotides Protocol - http://www.roche-applied-science.com/PROD_INF/MANUALS/InSitu/pdf/ISH_139-142.pdf
Description:Protocol for the identification of single bacterial cells using DIG-labeled oligonucleotides. Includes: Organisms and growth conditions; Cell fixation and preparation of cell smears; DIG labeling of oligonucleotides with DIG-ddUTP; In situ hybridization using digoxigenin-labeled oligonucleotides; Detection of DIG-labeled oligonucleotides with fluorescently labeled anti-DIG Fab fragments; Detection of DIG-labeled oligonucleotide.
Category:Cytogenetics Protocols: DIG Biotin Labeled Probe Protocols
Link Owner:
Date Added:December 14, 2007 09:38:56 PM
Number Hits:27
 

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