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DNA In Situ Hybridization with an Alkaline Phosphatase-Based Fluorescent Detection System - Details

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Protocols » Listing Details
ID:2011
Title: DNA In Situ Hybridization with an Alkaline Phosphatase-Based Fluorescent Detection System - http://www.roche-applied-science.com/PROD_INF/MANUALS/InSitu/pdf/ISH_124-126.pdf
Description:Protocol describes here a high sensitivity indirect detection procedure for DIG-labeled hybridization probes. The procedure uses the components of the HNPP Fluorescent Detection Set to form a fluorescent precipitate of HNPP (2-hydroxy-3-naphthoic acid-2’-phenylanilide phosphate) and Fast Red TR at the site of hybridization. Includes: In situ hybridization with DIG-labeled probes; Detection of DIG-labeled probes; Fluorescence microscopy.
Category:Cytogenetics Protocols: DIG Biotin Labeled Probe Protocols
Link Owner:
Date Added:December 14, 2007 09:34:53 PM
Number Hits:92
 

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