Special Feature

User Panel

My Panel

My Panel

Bookmark Science Articles

Recent News
Bookmark / Share This Science Site

Send Us Your Protocols

Assay of Intracellular Free Calcium in Suspended B Cells Protocol - Details

Link Details 

Protocols » Listing Details
ID:1932
Title: Assay of Intracellular Free Calcium in Suspended B Cells Protocol - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000011.pdf?pid=PP00000011
Description:This protocol describes a method to assess concentrations of free cytoplasmic calcium (Ca2+) in mouse splenic B cells in the absence and presence of ligands for cell surface receptors. This objective is accomplished with the Ca2+-sensitive fluorescent dye, Fluo-3, which permeates cells as an ester and is hydrolyzed in the cell to its Ca2+- sensitive acidic form.
Category:Cell Biology and Cell Culture: Flow Cytometry Protocols: Intracellular Ion Measurement Protocols
Link Owner:
Date Added:December 11, 2007 12:59:41 PM
Number Hits:10
 

Protocols Tags

  • alpha Protocols
  • antibodies Protocols
  • apoptosis Protocols
  • blunt Protocols
  • cell Protocols
  • cells Protocols
  • chromosome Protocols
  • cloning Protocols
  • cox Protocols
  • cryovials Protocols
  • culture Protocols
  • cyclooxygenase Protocols
  • death Protocols
  • describes Protocols
  • detection Protocols
  • dsrna Protocols
  • embryonic Protocols
  • end Protocols
  • free Protocols
  • freezing Protocols
  • have Protocols
  • hesc Protocols
  • how Protocols
  • human Protocols
  • hybridisation Protocols
  • labeling Protocols
  • mitochondrial Protocols
  • molecules Protocols
  • pcr Protocols
  • phage Protocols
  • plasmid Protocols
  • potential Protocols
  • preparation Protocols
  • presence Protocols
  • procedure Protocols
  • products Protocols
  • purified Protocols
  • reaction Protocols
  • reduce Protocols
  • restriction Protocols
  • rna Protocols
  • should Protocols
  • situ Protocols
  • stem Protocols
  • stocks Protocols
  • storage Protocols
  • that Protocols
  • thawing Protocols
  • titering Protocols
  • tunel Protocols


    •  

    Molecular Station Menu

    Welcome to Molecular Station!

    You have to register before you can post on our forums or use our advanced features. Register Now! Its Free and Fast!

    Already registered? Login now below.

    User Name:

    Password:

    Already registered and Forgot your password? Click below to recover it.

    Recover Lost Password

    Home
    Features

    Protocols

    DNA Forum

    Science Forum

    DNA Forum
    Biology Forum