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Assay of Intracellular Free Calcium in RAW 264.7 Cells Loaded with Fluo-3 Protocol - Details

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Protocols » Listing Details
ID:1930
Title: Assay of Intracellular Free Calcium in RAW 264.7 Cells Loaded with Fluo-3 Protocol - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000210.pdf?pid=PP00000210
Description:This protocol describes a method to assess concentrations of free cytoplasmic calcium, [Ca2+]i, in cultured adherent RAW 264.7 cells, using a 96- well plate format. This objective is accomplished by using the Ca2+-sensitive fluorescent dye, fluo-3, which permeates cell membranes as an ester and is hydrolyzed in the cell to its Ca2+-sensitive acidic form. Fluorescence for the adherent cells is measured over time by using a bottom read of a 96-well plate, with cells that have been washed.
Category:Cell Biology and Cell Culture: Flow Cytometry Protocols: Intracellular Ion Measurement Protocols
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Date Added:December 11, 2007 12:57:31 PM
Number Hits:88
 

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