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Assay of Intracellular Free Calcium in RAW 264.7 Cells for Ligand Screen Protocol - Details

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Protocols » Listing Details
ID:1929
Title: Assay of Intracellular Free Calcium in RAW 264.7 Cells for Ligand Screen Protocol - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000176.pdf?pid=PP00000176
Description:This protocol describes a method to assess concentrations of free cytoplasmic calcium (Ca2+) in cultured RAW 264.7 cells. This objective is accomplished with the Ca2+-sensitive fluorescent dye, fluo-3, which permeates cells as an ester and is hydrolyzed in the cell to its Ca2+-sensitive acidic form. Fluorescence is measured over time with adherent cells that have been washed free of extracellular dye.
Category:Cell Biology and Cell Culture: Flow Cytometry Protocols: Intracellular Ion Measurement Protocols
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Date Added:December 11, 2007 12:55:47 PM
Number Hits:60
 

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