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Simultaneous Analysis of DNA Content and Surface Immunophenotype Protocol - Details

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Protocols » Listing Details
ID:1928
Title: Simultaneous Analysis of DNA Content and Surface Immunophenotype Protocol - http://www.cyto.purdue.edu/flowcyt/research/cytotech/telford/ethanol.htm
Description:In an attempt to accurately measure DNA content with simultaneous preservation of cell surface markers, we have utilized gentle ethanol treatment techniques, which permeablize cells with minimal loss of surface antigen expression and antibody-antigen association. For some cell types, the presence of apoptotic cells based on reduced DNA content can also be detected. One such technique employs the addition of ethanol to cells previously resuspended in high concentrations of fetal bovine serum...
Category:Cell Biology and Cell Culture: Flow Cytometry Protocols: DNA Staining Protocols for Flow Cytometry
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Date Added:December 11, 2007 12:21:55 PM
Number Hits:26
 

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