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Protocol for Isolation, Cryopreservation, and Thawing of PBMCs - Details

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Protocols » Listing Details
ID:1810
Title: Protocol for Isolation, Cryopreservation, and Thawing of PBMCs - http://www.biomedcentral.com/content/supplementary/1471-2172-6-17-S1.pdf
Description:Cryopreserved PBMCs are a common specimen source for studies of immunological responses to vaccines, immunotherapies, etc. The health and viability of cells recovered post-cryopreservation are of course critical to the success and accuracy of immunological assays performed on them. This protocol standardizes PBMC isolation and cryopreservation techniques, specifically for the assessment of thawed cells by cytokine flow cytometry.
Category:Primary Cell Isolation and Culture Protocols: Mammalian Cell Culture Protocols
Link Owner:
Date Added:December 07, 2007 12:09:43 PM
Number Hits:342
 

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