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2-D Polyacrylamide Gel Electrophoresis Protocol - Details

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Protocols » Listing Details
ID:1795
Title: 2-D Polyacrylamide Gel Electrophoresis Protocol - http://cgap-mf.nih.gov/Protocols/DNARNAProteomicAnalysis/Proteomics/2DPage.html
Description:Protocol for 2-D polyacrylamide gel electrophoresis. Includes: 50 ml IEF Lysis Buffer; 10X Electrophoresis Running Buffer (10 L); 30% Acrylamide Stock (1 liter); Separating Acrylamide Gel; 50 ml Equilibration Buffer I; 50 ml Equilibration Buffer II; Transfer Buffer; Sample Preparation; Tissue Processing; Reswelling; Prepare Gradient Acrylamide Gel (9-18%); Transfer and Sequencing of Proteins.
Category:Primary Cell Isolation and Culture Protocols: Laser Capture Microdissection Protocols
Link Owner:
Date Added:December 07, 2007 11:11:25 AM
Number Hits:462
 

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