2-D Polyacrylamide Gel Electrophoresis Protocol - Details

Link Details

Protocols » Listing Details

ID:	1795
Title:	2-D Polyacrylamide Gel Electrophoresis Protocol - http://cgap-mf.nih.gov/Protocols/DNARNAProteomicAnalysis/Proteomics/2DPage.html
Description:	Protocol for 2-D polyacrylamide gel electrophoresis. Includes: 50 ml IEF Lysis Buffer; 10X Electrophoresis Running Buffer (10 L); 30% Acrylamide Stock (1 liter); Separating Acrylamide Gel; 50 ml Equilibration Buffer I; 50 ml Equilibration Buffer II; Transfer Buffer; Sample Preparation; Tissue Processing; Reswelling; Prepare Gradient Acrylamide Gel (9-18%); Transfer and Sequencing of Proteins.
Category:	Primary Cell Isolation and Culture Protocols: Laser Capture Microdissection Protocols
Link Owner:
Date Added:	December 07, 2007 11:11:25 AM
Number Hits:	807