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Passage of Embryonic Stem (ES) Cells Protocol - Details

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Protocols » Listing Details
ID:1712
Title: Passage of Embryonic Stem (ES) Cells Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/16/pdb.prot4401
Description:This protocol describes passage of ES cells. They should be split at 1:3 to 1:7 every 2-3 days depending on their growth rate when they reach 70% confluency. They should never be allowed to grow past 90% confluency, but rather they should form tightly packed colonies not touching each other.
Category:Stem Cell Protocols
Link Owner:
Date Added:December 06, 2007 03:01:08 PM
Number Hits:9
 

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