Special Feature

User Panel

My Panel

My Panel

Bookmark Science Articles

Recent News
Bookmark / Share This Science Site

Send Us Your Protocols

Preparing Feeder Cell Layers from STO or Mouse Embryo Fibroblast (MEF) Cells: Treatment with Mitomyc - Details

Link Details 

Protocols » Listing Details
ID:1703
Title: Preparing Feeder Cell Layers from STO or Mouse Embryo Fibroblast (MEF) Cells: Treatment with Mitomyc - http://www.cshprotocols.org/cgi/content/extract/2006/16/pdb.prot4399
Description:This protocol describes the preparation of feeder cell layers from MEF cells or from the STO mouse fibroblast cell line. The cells are rendered mitotically inactive by treatment with mitomycin C. The feeder layers can then be used to maintain embryonic stem (ES) cells in the undifferentiated state.
Category:Stem Cell Protocols: Feeder Cell Preparation Protocols
Link Owner:
Date Added:December 06, 2007 02:28:27 PM
Number Hits:72
 

Protocols Tags

  • 5´ Protocols
  • alcian Protocols
  • alternating Protocols
  • antibody Protocols
  • assays Protocols
  • binding Protocols
  • cell Protocols
  • cervical Protocols
  • chip Protocols
  • clones Protocols
  • collection Protocols
  • combined Protocols
  • cryogenic Protocols
  • describes Protocols
  • dislocation Protocols
  • dna Protocols
  • dsrna Protocols
  • early Protocols
  • electrophoresis Protocols
  • embryonic Protocols
  • embryos Protocols
  • field Protocols
  • gel Protocols
  • gels Protocols
  • human Protocols
  • immunoprecipitation Protocols
  • into Protocols
  • kinase Protocols
  • laboratory Protocols
  • library Protocols
  • ligation Protocols
  • microinjection Protocols
  • monoclonal Protocols
  • mouse Protocols
  • one Protocols
  • oocytes Protocols
  • pcr Protocols
  • promega Protocols
  • pulsed Protocols
  • shrna Protocols
  • staining Protocols
  • standard Protocols
  • stem Protocols
  • storage Protocols
  • tips Protocols
  • transformation Protocols
  • transverse Protocols
  • vector Protocols
  • via Protocols
  • vodkin Protocols


    •  

    Molecular Station Menu

    Welcome to Molecular Station!

    You have to register before you can post on our forums or use our advanced features. Register Now! Its Free and Fast!

    Already registered? Login now below.

    User Name:

    Password:

    Already registered and Forgot your password? Click below to recover it.

    Recover Lost Password

    Home
    Features

    Protocols

    DNA Forum

    Science Forum

    DNA Forum
    Biology Forum