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Isolation and Freezing of Primary Mouse Embryonic Fibroblasts (MEF) For Feeder Plates - Details

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Protocols » Listing Details
ID:1698
Title: Isolation and Freezing of Primary Mouse Embryonic Fibroblasts (MEF) For Feeder Plates - http://www.cshprotocols.org/cgi/content/extract/2006/18/pdb.prot4482
Description:This protocol describes the isolation of fibroblasts from mouse embryos. Mouse embryonic fibroblast (MEF) cells are used as a feeder layer for the culture of mouse embryonic stem (ES) cells to help maintain them as pluripotent stem cells. The inhibition of ES-cell differentiation provided by the MEF feeders appears to be due to their production of leukemia inhibitory factor (LIF).
Category:Stem Cell Protocols: Feeder Cell Preparation Protocols
Link Owner:
Date Added:December 06, 2007 02:23:15 PM
Number Hits:162
 

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