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De Novo Isolation of Embryonic Stem (ES) Cell Lines from Blastocysts Protocol - Details

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Protocols » Listing Details
ID:1692
Title: De Novo Isolation of Embryonic Stem (ES) Cell Lines from Blastocysts Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/16/pdb.prot4403
Description:The starting material for de novo isolation of stem cell lines can be either normal 3.5-days post coitum (dpc) expanded blastocysts or "delayed" blastocysts. Delayed blastocysts are usually collected 4-6 days after ovariectomy. For both groups of blastocysts, tissue culture procedures are similar. The only difference is the timing of the first disaggregation, because delayed blastocysts will initially grow more slowly.
Category:Stem Cell Protocols: Stem Cell Isolation Protocols
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Date Added:December 06, 2007 02:12:17 PM
Number Hits:50
 

 

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