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Differentiation of Embryonic Stem (ES) Cells Using the Hanging Drop Method - Details

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Protocols » Listing Details
ID:1679
Title: Differentiation of Embryonic Stem (ES) Cells Using the Hanging Drop Method - http://www.cshprotocols.org/cgi/content/extract/2006/18/pdb.prot4485
Description:In vitro differentiation of ES cells occurs when the cells are allowed to aggregate in suspension culture in the absence of mouse embryonic fibroblast (MEF) feeders and leukemia inhibitory factor (LIF). Hanging drops provide a uniform aggregate size, which is then expanded by continued growth in suspension culture. The embryoid bodies are then plated and allowed to differentiate further in culture.
Category:Stem Cell Protocols: Stem Cell Culture Protocols
Link Owner:
Date Added:December 06, 2007 01:49:16 PM
Number Hits:51
 

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