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Fixing Attached Cells in Paraformaldehyde Protocol - Details

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Protocols » Listing Details
ID:1651
Title: Fixing Attached Cells in Paraformaldehyde Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4294
Description:Treating cells with paraformaldehyde leads to the establishment of chemical cross-links between free amino groups. When the cross-links join different molecules, a latticework of interactions occurs that holds the overall architecture of the cell together. Commercial formaldehyde solutions are not recommended, because they lack the advantages of using a variable-length polymer, and the cells will simultaneously be fixed with the alcohol (usually methanol).
Category:Cell Culture Protocols: Cell Fixing Protocols
Link Owner:
Date Added:December 06, 2007 04:49:00 AM
Number Hits:949
 

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