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Fixing Attached Cells in Organic Solvents Protocol - Details

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Protocols » Listing Details
ID:1650
Title: Fixing Attached Cells in Organic Solvents Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/21/pdb.prot4293
Description:Organic solvents such as alcohols and acetone remove lipids and dehydrate cells, precipitating the proteins on the cellular architecture. Be aware that different antigens may be affected differently by the various solvents. If no previous data are available for your antigen, start with the 50/50 mixture. For tissue culture dishes, concentrations of acetone higher than 50% will destroy the integrity of the plastic.
Category:Cell Culture Protocols: Cell Fixing Protocols
Link Owner:
Date Added:December 06, 2007 04:48:01 AM
Number Hits:402
 

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