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Cryopreservation of Mammalian Culture Cells: Preparation and Recovery of Samples Protocol - Details

Link Details 


ID:1642
Title: Cryopreservation of Mammalian Culture Cells: Preparation and Recovery of Samples Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4350
Description:Protocol provides methods for cryofreezing and subsequent thawing of mammalian cells. Pre-confluent cells are trypsinized, pelleted, resuspended in freezing medium, and gradually frozen. When needed, frozen cells are thawed quickly under running tap water and transferred to growth medium.
Category:Cell Culture Protocols: Cell Storing and Cell Freezing Protocols
Link Owner:
Date Added:December 06, 2007 04:20:31 AM
Number Hits:88