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Lysing Tissue-Culture Cells for Immunoprecipitation Protocol - Details

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Protocols » Listing Details
ID:1641
Title: Lysing Tissue-Culture Cells for Immunoprecipitation Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4531
Description:For cells grown in tissue culture, the most useful method of lysis is treating with detergents, as described in this protocol. Non-ionic detergents, such as NP-40, solubilize the plasma and intracellular membranes, break many weak intermolecular bonds, and solubilize most of the commonly studied protein antigens. RIPA lysis buffer may be used as a more rigorous extraction buffer to release all but the insoluble proteins of the cell and to break most weak noncovalent interactions.
Category:Cell Culture Protocols: Cell Lysis Protocols
Link Owner:
Date Added:December 06, 2007 04:19:14 AM
Number Hits:306
 

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