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Detection of Mycoplasma in Mammalian Cell Cultures Using Fluorescence Microscopy Protocol - Details

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Protocols » Listing Details
ID:1634
Title: Detection of Mycoplasma in Mammalian Cell Cultures Using Fluorescence Microscopy Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4351
Description:Protocol describes a method for mycoplasma detection using Hoechst 33258, a fluorescent dye that stains DNA. Staining of particulate DNA matter around the cell nucleus (on the cell surface or in the cytoplasm) is indicative of mycoplasma contamination.
Category:Cell Culture Protocols: Cell Culture Contamination Prevention and Removal Protocols
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Date Added:December 06, 2007 04:04:35 AM
Number Hits:270
 

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