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Immunoprecipitation: Lysing Bacteria by Sonication Protocol - Details

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Protocols » Listing Details
ID:1631
Title: Immunoprecipitation: Lysing Bacteria by Sonication Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4532
Description:In this protocol, bacterial cells are lysed by being subjected to short, intense treatments with ultrasound, which breaks the cell walls and shears the DNA into sizes that will not affect the viscosity of the samples. Note that this method causes some denaturation of the samples. The resulting lysate is ready for preclearing.
Category:Bacterial Cell Culture Protocols
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Date Added:December 05, 2007 07:23:45 AM
Number Hits:302
 

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