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Extraction and Solubilization of Total Protein from Microorganisms Protocol - Details

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Protocols » Listing Details
ID:1630
Title: Extraction and Solubilization of Total Protein from Microorganisms Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4224
Description:The growth conditions of microbial cell cultures and the time of sample collection should be optimized and standardized when growing cells for protein extraction. Because cells may excrete proteases and other extracellular enzymes, and compounds in the medium may interfere with extraction, wash cultures with an isotonic buffer, such as PBS or sucrose before solubilization.
Category:Bacterial Cell Culture Protocols
Link Owner:
Date Added:December 05, 2007 07:22:52 AM
Number Hits:47
 

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