Special Feature

User Panel

My Panel

My Panel

Bookmark Science Articles

Recent News
Bookmark / Share This Science Site

Send Us Your Protocols

Maintenance of Probes in Bacteria Including Escherichia coli Protocol - Details

Link Details 

Protocols » Listing Details
ID:1629
Title: Maintenance of Probes in Bacteria Including Escherichia coli Protocol - http://wheat.pw.usda.gov/~lazo/methods/lazo/bactstor.html
Description:Plasmid (pUC series) containing genomic DNA fragments are maintained in E. coli strain DH5aTM. The E. coli cultures are routinely cultured at 37 C on Luria-Bertani (LB) agar on or in LB broth containing Ampicillin (30 µg/ml) or Carbenicillin (50 µg/ml broth, 100 µg/ml agar). E. coli strains are usually preserved in stab agar or glycerol for mid-term storage and lyophilized for long-term storage.
Category:Bacterial Cell Culture Protocols: Bacterial Stocks Protocols
Link Owner:
Date Added:December 05, 2007 07:20:44 AM
Number Hits:94
 

Protocols Tags

  • acrylamide Protocols
  • agarose Protocols
  • alkaline Protocols
  • allium Protocols
  • analysis Protocols
  • antigen Protocols
  • arms Protocols
  • bisulfite Protocols
  • buffers Protocols
  • capture Protocols
  • cdna Protocols
  • cell Protocols
  • cells Protocols
  • charged Protocols
  • conditions Protocols
  • dna Protocols
  • electroporation Protocols
  • elisa Protocols
  • flow Protocols
  • fragments Protocols
  • frozen Protocols
  • gels Protocols
  • gmpcpp Protocols
  • gtp Protocols
  • hiv Protocols
  • into Protocols
  • inverse Protocols
  • ligation Protocols
  • lines Protocols
  • membranes Protocols
  • neutral Protocols
  • nylon Protocols
  • optimal Protocols
  • other Protocols
  • p24 Protocols
  • pcr Protocols
  • polymerization Protocols
  • positively Protocols
  • purification Protocols
  • recovered Protocols
  • restriction Protocols
  • rna Protocols
  • sequencing Protocols
  • taxol Protocols
  • transfection Protocols
  • transfer Protocols
  • tubulin Protocols
  • vector Protocols
  • whole Protocols
  • yeast Protocols


    •  

    Molecular Station Menu

    Welcome to Molecular Station!

    You have to register before you can post on our forums or use our advanced features. Register Now! Its Free and Fast!

    Already registered? Login now below.

    User Name:

    Password:

    Already registered and Forgot your password? Click below to recover it.

    Recover Lost Password

    Home
    Features

    Protocols

    DNA Forum

    Science Forum

    DNA Forum
    Biology Forum