Bioinformatics, Protocols, DNA RNA Protein Proteomics
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Link Details
| ID: | 1629 |
| Title: | Maintenance of Probes in Bacteria Including Escherichia coli Protocol - http://wheat.pw.usda.gov/~lazo/methods/lazo/bactstor.html |
| Description: | Plasmid (pUC series) containing genomic DNA fragments are maintained in E. coli strain DH5aTM. The E. coli cultures are routinely cultured at 37 C on Luria-Bertani (LB) agar on or in LB broth containing Ampicillin (30 µg/ml) or Carbenicillin (50 µg/ml broth, 100 µg/ml agar). E. coli strains are usually preserved in stab agar or glycerol for mid-term storage and lyophilized for long-term storage. |
| Category: | Bacterial Cell Culture Protocols: Bacterial Stocks Protocols |
| Link Owner: | |
| Date Added: | December 05, 2007 07:20:44 AM |
| Number Hits: | 27 |
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