home > protocol-links > detail.php
You have to register before you can post on our forums or use our advanced features. Register Now! Its Free and Fast!
Already registered? Login now below.
Already registered and Forgot your password? Click below to recover it.
Join now - it's fast and free!
Molecular Station is THE largest network of researchers, scientists and science lovers anywhere!
A fact is a simple statement that everyone believes. It is innocent, unless found guilty. A hypothesis is a novel suggestion that no one wants to believe. It is guilty, until found effective. ~Edward Teller
Link Details
| ID: | 1629 |
| Title: | Maintenance of Probes in Bacteria Including Escherichia coli Protocol - http://wheat.pw.usda.gov/~lazo/methods/lazo/bactstor.html |
| Description: | Plasmid (pUC series) containing genomic DNA fragments are maintained in E. coli strain DH5aTM. The E. coli cultures are routinely cultured at 37 C on Luria-Bertani (LB) agar on or in LB broth containing Ampicillin (30 µg/ml) or Carbenicillin (50 µg/ml broth, 100 µg/ml agar). E. coli strains are usually preserved in stab agar or glycerol for mid-term storage and lyophilized for long-term storage. |
| Category: | Bacterial Cell Culture Protocols: Bacterial Stocks Protocols |
| Link Owner: | |
| Date Added: | December 05, 2007 07:20:44 AM |
| Number Hits: | 27 |