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Fractionation of Mitochondria, Lysosomes, Peroxisomes, ER and Golgi in Pre-formed Iodixanol Gradient - Details

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Protocols » Listing Details
ID:1591
Title: Fractionation of Mitochondria, Lysosomes, Peroxisomes, ER and Golgi in Pre-formed Iodixanol Gradient - http://www.axis-shield.com/densityhome/optiprep/S13.pdf
Description:Generally in iodixanol gradients the density of organelles decreases in the series: peroxisomes, mitochondria, lysosomes, ER, Golgi, although in Dictyostelium discoideum, the lysosomes are denser than the mitochondria. Iodixanol gradients can usually provide satisfactory resolution of all these membrane particles although it may be necessary to modulate either the gradient or centrifugation parameters in order to optimize a particular separation.
Category:Cell Organelle Protocols: Subcellular Fractionation Protocols: Mitochondria Isolation Protocols
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Date Added:December 04, 2007 06:18:31 AM
Number Hits:486
 

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