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Purification of Golgi Membranes from a Light Mitochondrial Fraction in a Self-Generated Gradient - Details

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Protocols » Listing Details
ID:1589
Title: Purification of Golgi Membranes from a Light Mitochondrial Fraction in a Self-Generated Gradient - http://www.axis-shield.com/densityhome/optiprep/S12.pdf
Description:This protocol uses a "light mitochondrial" pellet from a mammalian liver homogenate. The gradient thus has to resolve a variety of denser components (peroxisomes, lysosomes, mitochondria) from the Golgi membranes, which have a low density in iodixanol (1.06-1.09 g/ml) [1]. The protocol is specifically tailored to the purification of Golgi membranes from this pellet and is unsuitable for the isolation or analysis of other organelles present in the light mitochondrial fraction.
Category:Cell Organelle Protocols: Subcellular Fractionation Protocols: Membrane Isolation Protocols
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Date Added:December 04, 2007 06:04:12 AM
Number Hits:81
 

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