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Purification of Caveolae Membranes from a Plasma Membrane Fraction of Cultured Cells and Tissues - Details

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Protocols » Listing Details
ID:1588
Title: Purification of Caveolae Membranes from a Plasma Membrane Fraction of Cultured Cells and Tissues - http://www.axis-shield.com/densityhome/optiprep/S19.pdf
Description:The first part of the isolation procedure is a flotation through a continuous iodixanol gradient; this gradient is essentially a resolving gradient in which the caveolin-rich vesicles are concentrated in the top third of the gradient, while the predominantly caveolin-poor vesicles band in denser regions. A second discontinuous gradient is essentially a concentration gradient to band the caveolin-rich vesicles sharply at an interface.
Category:Cell Organelle Protocols: Subcellular Fractionation Protocols: Membrane Isolation Protocols
Link Owner:
Date Added:December 04, 2007 06:01:45 AM
Number Hits:194
 

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